This combination is then incubated at fifty five°C for 1 hour. Then the sample is phenol-extracted when with phenol/chloroform/isoamyl Alcoholic beverages solution and centrifuged. The aqueous layer is eliminated to some refreshing microcentrifuge tube. The DNA is ethanol-precipitated, re-suspended in buffer, and ethanol-precipitated a second time. After the pellet is https://www.pinterest.com/pin/993958580252567997/